The Florida Everglades a Wetlands Ecosystem Essay Example

The Florida Everglades a Wetlands Ecosystem Paper All Of the wildlife in the Everglades is totally dependent on the cycling Of water. One example of this dependence is the feeding relationship between the snail kite (an endangered bird species), and the apple snail (a freshwater mollusk the size of a golf ball) (Taller). The apple snails reproduce during the rainy season. When water levels are at their highest, they lay thousands of tiny pink eggs on the stalks of marsh grasses. As the water recedes, the snail kites fly all over the Everglades looking for them. Once they find them, they swoop down and use their specialized beaks to pluck the tender snails from heir shells. The water cycle and the lives of apple snails and snail kites are intertwined. Snail kites depend on the successful reproduction of apple snails, which is, in turn, affected by the amount of rainfall. Only recently have scientists been able to observe how close this relationship is. When humans drained large areas of the Everglades and converted them to agricultural lands, the population of apple snails decreased sharply. This had a dramatic effect on the snail kite population. In 2003, only 1 600 snail kites remained in Florida, the birds only U. S. Habitat (Smith). The relationship among humans, nail kites, and apple snails illustrates the delicate balance of nature in an ecosystem. When humans alter the water cycle, they directly affect the food chain. Alligators are animals that often come to mind when people think of the Everglades. The American alligator, once a highly endangered reptile, plays a critical role in the Everglades ecosystem, especially during the dry season (Alligator Holes). As winter approaches, water levels begin to drop. Alligators, which need an ample supply of water to survive, sense the changing of the season and begin to prepare for the dry months ahead. We will write a custom essay sample on The Florida Everglades a Wetlands Ecosystem specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on The Florida Everglades a Wetlands Ecosystem specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on The Florida Everglades a Wetlands Ecosystem specifically for you FOR ONLY $16.38 $13.9/page Hire Writer Using their powerful snouts, tails, and legs, they make comfortable dens for themselves by slashing small plants, and muck out of the marsh. As it thrashes its body from side to side, it creates a small hole filled with water. Plant matter and mud piled up around the edges of the hole create dry ground on which other plants eventually grow. After many years, grass, trees, and other plants surround these gator holes like fences. Gator holes are important to other species as well. As the water becomes scarce during the dry season, many animals search for food and remaining pockets of water. The gator holes attract crayfish, frogs, turtles, fish, and other aquatic species, all seeking refuge in the deeper waters of the gator holes. Muskrats, otters, deer, and raccoons, as well as a wide variety of beautiful birds, such as ibises, egrets, and herons, visit these sanctuaries to feed on the small animals that can be found there. Because alligators and the watery hollows they make play such an important role in the Everglades ecosystem, they are considered to be a keystone species since many other species depend upon them for their survival. This has earned them the nickname keepers of the glades. Due to constantly changing water levels, ecosystems like the Everglades can be very unpredictable places. Since the 1 8005, people have tried to control the Everglades to prevent flooding (Blake). Large canals were built to send the water into the ocean and away from the Everglades. The land along the canals dried up and became more useful to people. Before so much Of the Everglades was drained, most of its water came from Lake Cheekbone, which sometimes overflowed along its southern edge. With an annual rainfall of nearly 60 inches (Everglades) and the overflow from the lake, a large area f the Everglades used to be wet for most of the year. But the lake was a source of major flooding to towns, especially during the rainy season. In the 1 sass, other flood control projects were started, including the construction of a dike along Lake Cheekbones southern rim. Because the water no longer overflowed from the lake; farmers and ranchers now had more dry land on which to live and work. More projects followed in the asses and asses. Today, about half of the original Everglades has been drained to create dry land for towns and farms and much of the region is crisscrossed by an elaborate system of canals, dikes, and levees. Blake) Water control efforts have benefited the residents of south Arid, but now nature no longer controls the flow of water into the Everglades; as a result, the natural balance of the ecosystem has been damaged. The draining of the Everglades has harmed many animals that depend on water for reproduction, such as snails, fish, and frogs. Because these creatures are at the bottom of many Everglades food chains, their diminishing numbers have had a rippling effect throughout the entire community. Like the snail kite, other bird species C such as the ibis, heron, and the endangered wood stork CO have suffered. In fact, scientists have estimated that some bird populations have dropped about 90 percent (Birds) over the past fifty years because of the low water levels. Now scientists are encouraging us to realize that a great number of plants and animals must survive to help maintain this delicate environment. Because the canals and dikes have helped to dry up the land, part of the original Everglades has become a rich agricultural area. Yet, productivity within this marsh has had a negative effect on its wildlife. In the asses and asses, bald eagles and pelicans in the Everglades were among the many birds heartened with extinction by the chemical EDT. (Scott) Farmers sprayed EDT on their crops to control insects. They didnt realize that from heavy rains was washing the poisonous chemical into the Everglades. Scientists discovered that EDT caused the shells of birds eggs to thin, resulting in the death of many young birds before hatching. The U. S. Government finally banned the use of EDT in 1972. Agricultural runoff disrupts the Everglades ecosystem in other ways too. Fertilizers, which contain plant nutrients, are washed from the sugarcane plantations a few miles north. These fertilizers cause an excessive growth Of algae. The algae can form large mats called algal blooms, which float on the surface of the water and results in transportation. The effects of transportation can be seen as far south as Florida Bay. As the algae die and decompose, they use up large amounts of oxygen in the water, which causes fish, crabs, shrimp, insects, and other aquatic species to suffocate in the oxygen-depleted water. With the drier conditions created by flood-control, brush fires began to sweep through the Everglades in the 1 sass and asses. These devastating blazes led environmentalists to pressure the government to establish the Everglades National park. (Park Establishment) Today, visitors can experience Florists diverse, rare, and beautiful wildlife in the Everglades National Park. Located in the southwestern portion of the marsh, this is one of the largest national parks in the United States. Each year, millions of tourists come to see the huge array of tropical wildlife, which includes nearly six hundred different types of animals, such as alligators, crocodiles, pelicans, snakes, and a multitude of insect species. The Everglades is the largest freshwater wetlands in the continental United States and one of the worlds great biological treasures. It is home to many species of endangered plants and animals. But it is also an ecosystem in trouble. Over the past century, about half of the original Everglades has been drained, filled, and converted for farmland and other development. Much of the nutrient-filled water that once flowed naturally through the Everglades has been artificially diverted to sugarcane plantations. As a result, the entire ecosystem has suffered. Damaged wetlands cannot provide suitable habitat for the plants and animals that depend on it for survival. In 1 983, the state of Florida, along with several environmental groups, munched the Save Our Everglades campaign to start looking at ways to preserve the troubled wetlands. The project had one clear goal: to make the Everglades look and function more like it did in 1900 than it did in 1983. Throughout the 1 sass, scientists worked on this plan. In 1 994, the Arid state legislature passed the Everglades Forever Act (Longboat), which authorized the Everglades Construction Project the largest effort ever attempted to restore an ecosystem. One of the projects in the Everglades restoration effort is the construction of 40,000 acres of artificial marshes (Diabetes). These marshes will serve as a protective barrier between the sugarcane plantations and the rest of the Everglades ecosystem. The artificial wetlands are essentially huge ponds surrounded by dirt and filled with lots of nutrient-loving plants. Scientists hope that the plants will clean the water by catching and filtering out pesticides, fertilizers, and other farm runoff before it reaches the Everglades. The clean water will then be redirected back into the marsh. Another project is to restore the Kismet River (Restoring a River). Before people began tinkering with the Everglades, the Kismet was a 102-mile meandering river that supplies most of the water to Lake Cheekbone. In 1961, engineers straightened the Kismet River to control flooding around Lake Cheekbone and to make room for farms. Most of the water in the river was then diverted into a 55-mile straight channel, officially named the C-38 canal. Scientists expect that once the river IS returned to its original path, the habitat for more than three hundred fish and wildlife species, including the endangered wood stork and snail kite, will be restored. A total of 24 endangered or threatened plant and animal species live in the Everglades ecosystem (Threatened Endangered Species) and most of these species face extinction due to habitat loss. One large Everglades mammal that faces extinction due to habitat loss is the Florida panther, a type of cougar. Two hundred years ago, cougars roamed freely from Alaska to South America; but today, due to habitat loss, the only cougar population east of the Mississippi River is the Florida panther. Despite widespread efforts to save the panther, fewer than fifty remain in the wild (Florida Panther). This makes it one of the most endangered animals in North America. If habitat loss can be halted, biologists hope that the panther population will increase.

Filo Essays - Ondertitel, Basis Van, Ervaring, Manier, Kies, Rust

Filo Essays - Ondertitel, Basis Van, Ervaring, Manier, Kies, Rust Filo Essayopdracht over hoofdstuk 3 Kennis en Weten Schrijf een essay onder de werktitel Kennis ? een venster op de werkelijkheid of een product van mijn geest? Je presenteert in dit essay twee filosofische theorie?n over het proces van kennisverwerving en legt uit op welke punten ze van elkaar verschillen. Kies hiervoor een rationalist en een empirist. Vervolgens leg je aan je lezer uit waarom de ene theorie volgens jou een overtuigender antwoord biedt op de gestelde vraag dan de andere. Schrijf je essay op basis van de hierna volgende richtlijnen. Beperk je bij het schrijven tot n gedachte per alinea. Begin met een eerste versie. De verfraaiing ? stilistisch en anderszins ? komt daarna pas. Je kunt rust en systeem in je schrijven brengen door je werkstuk alvast in te delen overeenkomstig de hieronder volgende 8 punten. Kennis ? een venster op de werkelijkheid of een product van mijn geest? 1Werktitel Als je klaar bent met het schrijven van je essay kun je de werktitel desgewenst vervangen door een eigen titel, eventueel met een ondertitel. Een goede titel is zowel zakelijk als pakkend: hij dekt de lading en spreekt de lezer direct aan. 2Inleiding en probleemstelling -Begin je essay met een levendige schets van een concrete ervaring of situatie die je lezer op een directe manier met je onderwerp confronteert. Welke ervaring of situatie je hier schetst is aan jou. Eis: je schets moet aansluiten op de probleemstelling. -Vervolgens formuleer je op basis van deze schets het probleem dat je aan de orde wilt stellen: we menen dat onze kennis betrekking heeft op de werkelijkheid, maar kunnen we daar wel zo zeker van zijn? Slaat datgene wat we weten op de werkelijkheid zelf of is onze kennis slechts een product van onze eigen geest? 3Aankondiging Nu schets je hoe je deze vraag gaat uitwerken. Je geeft aan: -welke filosofen je achtereenvolgens gaat bespreken en vergelijken; -om te kunnen zien wie het meest overtuigende antwoord biedt op je probleem; -eventueel kun je alvast vooruitlopen op de conclusie, maar om de spanning erin te houden kun je die ook in het midden laten. 4Filosoof 1 -Geef hier een uitwerking van de wijze waarop deze filosoof het proces van kennisverwerving beschrijft. Doe dit met het oog op de vraag die je in de inleiding hebt geformuleerd: kunnen we wel zo zeker zijn dat onze kennis betrekking heeft op de werkelijkheid en niet slechts een product is van de geest? Dat betekent dat je die punten moet accentueren waaraan je de argumenten voor een antwoord op deze vraag kunt ontlenen. -Benoem stilzwijgende vooronderstellingen, principi?le uitgangspunten en formuleer aan de hand daarvan eventuele vragen en/of bezwaren, uiteraard alleen als ze relevant zijn voor je centrale vraag. -Eindig deze paragraaf met een tussenconclusie: hoe zou deze filosoof je vraag beantwoorden? 5Filosoof 2 -Verwerk hier de wijze waarop de tweede filosoof het proces van kennisverwerving beschrijft. Ook hier uiteraard de vraag uit de inleiding in het oog houden: betreft kennis de werkelijkheid zelf of is het slechts een product van de geest? Weer accentueer je de punten waaraan je argumenten kunt ontlenen. -Benoem weer eventuele vooronderstellingen, principi?le uitgangspunten en formuleer aan de hand daarvan relevante vragen en/of bezwaren. -Besluit opnieuw met een tussenconclusie: welk antwoord zou filosoof 2 geven op je uitgangsvraag? 6Vergelijking en kritische beoordeling Beschrijf puntsgewijs eerst de overeenkomsten en vervolgens, op basis daarvan, de verschillen tussen beide filosofen. Laat hier duidelijk je eigen stem horen: jij bent degene die uitmaakt welke van de twee filosofen je het meest overtuigt en waarom. De bij 4 en 5 geformuleerde vragen en bezwaren kunnen je hierbij behulpzaam zijn. Werk in deze vergelijking toe naar de conclusie. 7Conclusie Keer nu uitdrukkelijk terug naar de hoofdvraag. Op basis van de deelconclusies en de vergelijking formuleer je jouw antwoord op de beginvraag. Je essay hangt in de lucht als je nalaat een antwoord te formuleren. Dit antwoord: -is helder en ondubbelzinnig, bijvoorbeeld: - ja, onze kennis betreft de werkelijkheid zelf - nee, kennis is slechts een product van onze geest. - anders, namelijk?; -vat kort de doorslaggevende argumenten samen; -geeft geen nieuwe informatie. 9Ten slotte Aardig is om aan het slot terug te komen op het voorbeeld uit het begin, maar noodzakelijk is dit niet. Beoordeling De volgende aspecten van je essay wegen mee bij de beoordeling: -logische opbouw (dus houd je zoveel mogelijk aan bovengeschetste opbouw); -concreetheid (geef voorbeelden); -kritische bespreking van vooronderstellingen en uitgangspunten (onder andere in je

17 Writing Tips Actionable Exercises to Write Better Today [VIDEO]

17 Writing Tips Actionable Exercises to Write Better Today [VIDEO] 17 Writing Tips Actionable Exercises to Write Better Today [VIDEO] Writing tips have aided every writer out there- from Ernest Hemingway to Stephen King.And now you’re here for a reasonYou want to learn how to write better through specific writing tips. Because lets be honestwe all feel like our writing could use some improvement.What you didn’t know is that you’ll learn a whole lot more than that by reading this post and you’ll find out exactly what if you stick with us.Writing is a skill you can never be the â€Å"best† at. You will always be able to grow and expand on your writing skills. Once you’ve reached what you believe is your very best, there is still mountains more you can improve upon.That’s part of the magic of being a writer.But it can be hard to know where you actually need the improvement. Which areas are your weakest and which do you excel in?Here are 17 strong writing tips:Write what you want to read Write with intention Use psychologyWrite as often as you can Eliminate distractions Research storytelling and story structure Always get feedback Focus on new ways to phrase common visuals Practice writing when you’re not writing Use strong language Just write to write Just do it. Youve got to work. Write for yourself first. Quantity will make up for quality. Tell the truth. You cant edit a blank page.Its one thing to improve your grammar, its another to work on bettering the actual writing.If you’re like me (and almost all writers out there), you likely struggle with insecurity in your writing. Us writers have a tendency to focus on the bad without knowing how to make it better, and this can often cost us our writing motivation.NOTE: We cover a number of writing tips in our VIP Self-Publishing program, along with everything youll need to write, market, and publish your book to bestseller status.Click here to learn moreLet’s get started. Writing Tips to Help You Become an AuthorIf you’re looking for a way to get your book done quickly a nd with quality, you’re in the right place.We put together this free training for you to learn exactly the writing tips that helped Chandler Bolt hit bestseller status with all 6 of his books. Join your FREE training and learn how you canwrite abetterbook in as little as 90 days if you really focus.Just click the button below to watch!Click here to start your training TODAYHow to Improve Writing with Tips for Writing a BookIn order to improve your writing skills, you have to commit to writing as much as you can, using different writing exercises, and reading often. You have to form a writing habit in order to do this.But there is good news about this.Your writing skills are not stagnant. They change and grow as you do.Think of it as running. The more you run and train, the better you become. It can be really hard to write a book at first but as you learn new techniques, how to use literary devices, and new methods for making it easier, you become a stronger, better runner.Wr iting is exactly the same.The way you improve your writing skills is by making a commitment to you, your work in progress, and all the people who can benefit from your book.How do You Become a Good Beginner Writer?Being a good beginner writer is about learning the craft of writing and learning specific techniques that make writing good in the first place.In fact, becoming a good beginner writer is all about reading as much as you can and writing as much as you can. This is what will help you recognize those literary elements you can then replicate and make your own when writing and editing.Just like I mentioned above, the more you can write, the better you will get, and this makes publishing your book and showing it to the world much easier.But it’s also about consuming content about becoming a better writer, like podcasts, blog posts, and videos around the craft of writing.These are our favorite writing tips resources:The Self-Publishing School Youtube ChannelOur Podcast, wh ere we highlight success stories and learn how authors made it happenJenna Moreci’s Youtube channel featuring the best fiction and self-publishing writing tipsDailyWritingTips.com, a blog featuring unique and specific tips for writingHannah Lee Kidder’s Youtube channel including tips from a multi-published fiction authorThis Stephen King video featuring his own tipsBrandon Sanderson’s lectures from a college classroomChandler Bolts personal Youtube channel for productivity advice and moreJustPublishingAdvice.com, a blog dedicated to sharing helpful publishing and writing tips.What are some writing tips for beginners?Being a newbie writer is not easy. These are some of the top writing tips we suggest in order to improve your writing skills as a beginner.Writing Tip #1 Write what you want to readIf you yourself wouldn’t pick up the book or story you’re writing and read it with joy, then you shouldnt’ be writing it.â€Å"But what if I think o ther people will like it even if I don’t?†This is a very common argument against this writing tip but it’s not sound. And the reason for that is because you’ll lack the passion.When you create a story that you love yourself, it comes through in the writing. It’ll read as if the words and your protagonist and characters as a whole pop off the page instead of lying flat.It will also be much easier to write and you’ll want to write it more than if you didn’t enjoy the story or topic as much.So for this writing tip, ask yourself these questions:Would you pick it up to read the back cover?Would you personally look for a book like this?Is this a book genre you personally enjoy?Will you develop the characters in a way that makes you root for them?Is the story structure captivating to you?Have you read and loved other books with similar worlds/characters/stories?If you cant answer these questions with a confident yes, skip the book idea and wr ite one you actually want to.Writing Tip#2 Write with intentionAll writing has a purpose and it needs a purpose if you want your writing to get better and read as something enjoyable.When you have a reason for writingwhat you’re writing, it becomes so much easier and it feels like you’re fulfilling a purpose rather than just writing a book.If all youre doing is writing a book to make money, then your heart (and therefore your passion) is in the wrong place. This makes it very clear to readers through your writing.Below is a writing tips exercise to help you achieve writing with intention.Writing Tip#3 Use psychology to write betterYes, there is research involved no matter what kind of book you’re writing.â€Å"But how can psychology actually help my writing improve?†In order to craft your book in a way that speaks to readers how you intend it to, you have to understand how the human mind works.This is how using psychology as a writing tip helps you get better:Youll craft more realistic charactersYour antagonists and protagonists motives will be more realisticYou can take your readers on a better experience by learning to manipulate their emotions with your plotYou can easily hit emotional triggers in readers that prompt them to keep turning pagesYoull better understand what it takes to write a novel thats engagingThe Write Practice has a fantastic resource for how to use psychology to become a better writer.Once you know how people interpret different events, messages, and themes, you can weave them into your book so it has more impact when they’re finished reading.And for the fiction writers out there, psychology helps you create real and lifelike characters that leave readers itching to turn that page and read more about them and their journey.Writing Tips Action Step:In order to accurately research for your book, think about what you want your readers to take away from each chapter, and then the book as a whole.Then res earch how real people interpret those specific messages.For example:If you want readers to feel inspired during a certain part of your book, research â€Å"psychology of inspiration† and read how one can build up to feel inspired and even how it affects their outlook in order to better craft the next chapters.Writing Tip#4 Write as often as you canEven if all you’re writing is a paragraph, it’s better than not writing at all.And if you can’t add on to your book for whatever reason (maybe a lack of an outline?), then write something else.Here are a few ways you can utilize this writing tip by writing something else:Write a short storyStart a new novelWrite a poemSkip to a new section in your book to writeWrite about your life in prose to practice descriptionsThe point is to write as often as you can because the more you write, the better you will get. It will help you pinpoint weaknesses in your writing and you’ll notice improvements as you write.W riting more often also allows you to flex your imagination, which is indeed much like a muscle. The more you use it, the stronger it gets and therefore, you’ll be able to write with more creativity.Writing Tip#5 Eliminate distractionsIn this age of technology and helpful writing software, there are endless amounts of distractions.We almost always have our phones within reach, a computer right at our fingertips (literally, if you’re writing), and a TV nearby with access to Netflix, Hulu, and other attention-sucking programs.If you want to write better, you have to eliminate distractions that keep you from writing.Here are our writing tips to get rid of distractions:Use a distraction-blocking App like Freedom or PauseForShut your phone off and put it in another roomClose out of all apps or windows on your computerSpend 15 minutes listening to music that reminds you of your book to get you in the zoneTell all your friends/family to leave you alone for writing timeAs ment ioned above, the more you write, the better you get. But you can’t write if you’re constantly checking your phone, email, or watching TV.Writing Tip#6 Research storytelling and story structureThis is largely for the fiction writers out there, but all writers can benefit from this writing tip of improving your storytelling.Storytelling and writing are not the same things.Writing is the way in which you describe what’s happening within the story. The story itself is a whole other piece of the puzzle and is arguably the most important piece.When you have a story idea worth writing, theres a few things to remember.Here are our top writing tips for learning the craft of storytelling:Study comedians the reason comedy is, well, funny is because comedians know how to tell stories in a way that keep us on the edge of our seat, and then they surprise us, which often initiates the laughter.Learn from great storytellers Stephen King is one of the best storytellers of al l time. He has a book, On Writing, that touches on this craft. Give it a read for some of the best writing tips youll find.Read as much as you can Writers learn how to write through reading. The more you read, and the wider variety of genres, the more youll naturally pick up on the art of storytelling.Get feedback on your stories This is the hardest, but most crucial writing tip to help you improve. You have to understand your weaknesses in order to make them stronger. Ask friends and family for help in order to learn how to make your stories better.Writing Tips Action Step:Read books, listen to podcasts, or watch videos about the art of crafting a story.Another great way to learn the ins and outs of storytelling is to watch great comedians. The reason they can make you laugh is how they craft what they’re saying.Notice the pauses, when they speed through what they’re saying, and how they deliver that final line.These are all techniques you can use on a larger scale when writing your book.Writing Tip#7 Always get feedbackThis will always be the hardest, but most important part of improving your writing. Of all the writing tips to take and execute, this is the best one.It’s very difficult to gauge your own writing because you wrote it.This is much like trying to tickle yourself. It just doesn’t work because you’re the person doing it and is much more effective when someone else does it.Thats why the beta reading process is so vital. Its when you let others read your book in order to gain feedback from people in your intended audience.That’s what it’s like for your writing. You need an outside set of eyes on your work.Jenna Moreci has a great resource on the beta reading process you can check out.Here are some specific questions to ask others for this tip to improve writing:Did you find anything confusing or unclear?Did you understand why InsertNameHere did what they did?Were you able to easily follow the dial ogue?Was the dialogue in writing clear and concise?Which character did you empathize with more?Do you have any predictions about what will happen?Do you have any feedback I didnt ask you about?Writing Tip#8   Focus on new ways to phrase common visualsOne of the best ways you can strengthen your creativity is by consciously thinking about how you can describe common things in new, interesting ways.You want to make people see that common item or situation or visual in a brand new light.The way you can do this is to pause when you’re describing something in your writing and think to yourself, â€Å"how else can I explain this to create a stronger emotional impact?†Here’s an example of this writing tip if you’re still a little confused:â€Å"The sun set behind the trees and the world fell quiet.†Is this a bad way to describe a sunset and night beginning? No. However, you can easily get more creative about how to illustrate this to readers through wo rds. Like this:â€Å"Night yanked the horizon over the sun, silencing the world with its absence.†This is saying relatively the same thing, but in a way that stops and makes someone appreciate the way in which it was crafted.Writing Tip#9 Practice writing in your headThis might sound a bit confusing, so let me elaborate.When you look at the world, how do you see it? Probably the same way everyone else does.Here’s an example of how you can practice writing but only in your own head. This can help you learn how to craft your prose to read in a beautiful, elegant fashion while also being unique and interesting to readers.Right now, I’m looking out my window into the backyard. It has snow, the trees are bare, and the sky is a muted gray at the horizon, fading to a very faint blue as you look higher up.This is a very typical visual for winter (especially in Wisconsin).Now, in order to practice writing without writing, all you have to do is start describing what you see in prose that you would write in your own head.Like this:â€Å"Stillness hung in the air thicker than Christmas morning eggnog, the ground covered in a thin sheet of white speckled with brown where the snow failed to make its mark. Bare branches reached toward the absent sun, reluctantly accepting the gray of winter in its place.†This example is more prose than reality, but this is how you can sharpen those skill by just thinking in this way.Notice the world around you in the way you would write it in a book.The more you practice this when you’re on the subway, making dinner, or even watching your family and friends interact, the easier it will be to write those situations in your book.Think like a writer in order to become a better one.Writing Tip#10 Use strong languageThis writing tip can completely transform your writing for the better. It’s the single best way to make your writing more captivating without really adding anything new. You just simply hav e to replace weak language with stronger, more descriptive writing.This can take some time to get used to but the more you do it, the easier it will get.So how do you recognize weak language?Here are some mistakes to look for in your writing to utilizing this writing tip:Passive voice Passive voice is any use of a to be past participle. Now, thats just a fancy way of saying that if you have something was done by something, its passive voice. An example of this is: The chicken was beheaded by the farmer. That is passive voice, whereas, The farmer beheaded the chicken. is active voice.Weak verbs These are the basic, non-detailed version of better verbs. An example would be, She walked to the store. In this case, walked is the weak verb. You can use another form of this verb to create a stronger visual for your reader. Heres what that would look like: She strutted to the store.Emotion explaining Using words that are emotions in your writing is a pretty clear indicator you have to sh ow and not tell. Saying, She was scared, is telling. You can create a better experience for the reader by showing that shes scared through body language, dialogue, and description.We even make it simpler for you with our strong verbs list. It has over 200 strong verbs and includes the common weak verbs you can replace.Writing Tips Action Step:Fill out your information for instant access to your strong verbs list of over 300+ verbs to use! hbspt.forms.create({ portalId: "4208601", formId: "c14c8b05-e824-459b-b7eb-a03c87d82b93" });Writing Tip#11 Just write to writeForget about your goals. Forget about how anyone else will interpret what you’ve wrote and just write.Do it for you. Write what you like and what makes you happy.Don’t think about the future or publishing or where you’re going from here. Just grab that outline, sit down, and write because it’s fun.Believe it or not, this frees up a lot of mental space and allows you to write without thinking too much, which often helps you write better.One of the best writing tips I ever received was to always have a side project going on, something you have no intention of ever publishing. This is where your real writing happens.Its a place for you to experiment, discover your writing voice, and learn what you truly love to write while still working on your main project and accomplishing those goals.Writing Tips from Famous AuthorsWhat better way to improve your writing than to practice writing tips from those who have mastered the cr aft?Here are our top writing tips from professional writers like Stephen King, JK Rowling, and even Margaret Atwood.#1 â€Å"Just do it.†Much like we mentioned above, Margaret Atwood is a huge advocate of diving right in and just writing, despite your fears, insecurities, or lack of direction.â€Å"I think the main thing is: Just do it. Plunge in! Being Canadian, I go swimming in icy cold lakes, and there is always that dithering moment. ‘Am I really going to do this? Won’t it hurt?’ And at some point you just have to flop in there and scream. Once you’re in, keep going. You may have to crumple and toss, but we all do that. Courage! I think that is what’s most required.†As someone who has made waves with a number of her novels, including the masterpiece that landed her an entire TV series, The Handmaid’s Tale, she is someone you want to take advice from- especially now that Margaret Atwoods Masterclass is available.#2 Youve got to work for it.Much to every writer’s dismay, books don’t actually write themselves. If there was a special machine we could plug into our brain that would spit out a perfect copy of the story inside our minds, we would all opt for that instead of sitting down and plucking away at the keyboard.But that’s not a reality (at least not yet).Someone who knows the value of hard work when it comes to writing is J.K. Rowling. Perhaps you’ve heard of her?â€Å"Youve got to work. Its about structure. Its about discipline. Its all these deadly things that your school teacher told you you needed†¦ You need it.As hard as it can be, Rowling’s advice is as sound as any. Work for your book. Work hard so others can benefit from the worth you’re holding onto.#3 Write for yourself first.Stephen King has an entire memoir-ish that doubles as writing tips simply because writing has been nearly his entire life. One of the best lessons King says he ever lea rned was from a newspaper editor he worked for while he was in high school (which he discusses in his memoir/writing book On Writing) and he has maintained that voice in his head throughout each work he writes.When you write a story, you’re telling yourself the story. When you rewrite, your main job is taking out all the things that are not the story. Your stuff starts out being just for you, but then it goes out.On Writing by Stephen King continues to be a source of inspiration and help for writers everywhere. King has a way of pulling you in and giving you the BS-free advice all writers want and, in most cases, desperately need.#4 â€Å"Quantity will make up for quality.†Ray Bradbury is one of the most quoted authors out there. He shares his methods for writing and how to actually succeed in this industry.His best advice, in my opinion, comes from his book Zen in the Art of Writing, where he says you have to schedule the time to write and write daily because quant ity will make up for quality.In fact, quantity is what leads you to quality.Michelangelo’s, da Vinci’s, Tintoretto’s billion sketches, the quantitative, prepared them for the qualitative, single sketches further down the line, single portraits, single landscapes of incredible control and beauty.†In essence, the more you practice writing, the better youll become and that makes all the difference when it comes to separating yourself form other writers.#5 â€Å"Tell the truth.†Miss Angelou is an inspiration to writers everywhere. She’s a personal favorite of mine and her quotes and advice for both writing and life has always spoken to me on a different level than others.One of the best writing tips I’ve read of her is the fact that you have to write the truth.â€Å"I look at some of the great novelists, and I think the reason they are great is that they’re telling the truth. The fact is they’re using made-up names, made-up people, made-up places, and made-up times, but they’re telling the truth about the human being- what we are capable of, what makes us lose, laugh, weep, fall down, and gnash our teeth and wring our hands and kill each other and love each other.†When you have a truth worth sharing, writing becomes easier, more meaningful, and therefore more impactful for those reading it.This ties into our writing tip above about writing what you want to read. Focus on telling your truth.#6 â€Å"You can’t edit a blank page.†Are you sensing a theme within these writing tips yet?Even Jodi Picoult agrees that you can’t become a better writer if you never write.â€Å"You can always edit a bad page. You can’t edit a blank page.†The best of all writing tips is this one. You have to actually write if you want to get better because the great writing doesnt happen on the first try. It happens on the second, fifth, and even tenth.You first have to write the wo rds in order to make them better.Writing Tips to Get You Started TODAYIf you’re here, it means you’re ready to take the leap and start writing.We can even help you have your book outlined today but only if you take action now.

Analysis of the figuritive language in th Poems Richard Cory by Edwin Essay

Analysis of the figuritive language in th Poems Richard Cory by Edwin Arlinton Robinson and Paul Simon - Essay Example Applying these criteria in the two versions of Richard Cory would help decide which of the two poems is more deserving of merit. Published in 1897, Richard Cory by Edwin Arlington Robinson is the original version of the poem. Robinson depicts the despair and death of a wealthy man in his poem. Basically, the language used is folkloric. A folklore is an oral tradition handed down from one generation to another. Thus, the character of Richard Cory and his experience appears to be a hearsay, a myth. The linguistic elements of the poem support this idea. First, the lines of the poem strictly follow a common rhythmic pattern with the first and third lines and the second and fourth lines rhyming. This may seem awkward when reporting about suicide as it negates the natural utterance of a suicide narrative. Second, the line of thought makes the language appear climactic, thus making the poem appear too literary and stilted. Like any narrative, the poem starts with the introduction of the cha racter, thus in the first stanza, Richard Cory is described as â€Å"a gentleman from sole to crown, / clean-favored and imperially slim† (l. 3–4).

To what extent can russian agriculture companies develop and compete Essay

To what extent can russian agriculture companies develop and compete - Essay Example asing flows of labor.†2 However, it should be noted that globalization is not solely economic in nature but that it also includes cultural, technological, educational and other similar forms of exchanges among nations. Being such, it can be claimed that globalization plays a very integral role in the creation and understanding of the economic impetuses and policies of each and every country in the world today. And it is in this sense that Russia today is no different. It too since the reform period of 1990 to the present is trying to integrate in its economic policies and strategies the demands made by globalization. And this effort is congruent with the sentiment of experts in the fields of economics, business and politics who hold the idea that a global â€Å"exposure may help make the Soviet economy more efficient†3 In recognition of the vast potentiality of Russia in the global market, the researcher deems to look specifically into Russian agricultural sector focusing on AGRICO’s role in the further development of Russian agricultural sector both in local market and in the global sector. And this is done with the hope that despite the humongous problems within the sector which is rooted historically, Russian agriculture together with AGRICO manifest fundamental role of agriculture in Russian economy in particular and in the global economy in general. As stated earlier, this research will primarily focus on Russian agricultural sector with AGRICO as its focal point. In order to be able to clarify the pivotal role of AGRICO in understanding Russia’s effort in achieving global competitiveness in the agricultural sector of the global market, there are four questions which will be addressed. These are: Being such, this research will not touch on the entire Russian economy and politics but will zero-in only its agricultural sector, the researcher does not deny the interconnectedness of all the industries within the economy but for the purpose of

Investigating Glue Essay Example for Free

Investigating Glue Essay Title To recycle the bad protein food by reproducing glue Aim Have you even thrown some food because it becomes bad? Do you think it really waste money? Do you want to make them become useful again? If you do, our experiment will show you how to do and everything about it. Apparatus: Heater, measuring cylinder à ¯Ã‚ ¿Ã‚ ½2(250ml50ml), pH paper,beakerà ¯Ã‚ ¿Ã‚ ½2(300ml500ml),glass rod, spoon Chemical: skim milk, vinegar Abstract The sticking properties of different proteins in daily food are being investigated in our experiment. Casein in dairy products and textured soy protein (TSP) in soybean products are abstracted out by precipitation in acid. The samples are then neutralized by carbonate and undergo a series of stress tests to compare the strength of our reproduced protein glues with commercial products. The basic principle of the sticking properties of protein glues is the ionic attractions and the Van der Walls attractive force generated by the protein molecules. Protein molecules are highly folded, resulting in a great contact surface area and exposure of the hydrophobic bonds. When two surfaces of substances like wood or paper are held together by protein, linkages are formed in between the molecules, and the two surfaces are stick together and are difficult to separate. We expect the result the casein in milk and textured soy protein in soybean milk have comparable sticking strength as starch contained in commercial glue. The significant advantage of the production of our protein glues is that proteins in expired food can be recycled and reused to make the useful glue. The glue produced is also not toxic or harmful to the environment. Introduction 1)The history of glue The earliest evidence of use of glue can still be observed in the cave paintings made by Neanderthal ancestors in Lascaux, France. These early artists wanted their work to last and mixed glue with the paint they used to help the colors resist the moisture of the cave walls. Egyptian artifacts unearthed in their tombs show many uses of glues; perhaps the most striking are the veneers and inlays in wood furniture, which was made using glue as early as 3,000 B.C. The Egyptians also used glue to produce papyrus. Greek and Roman artists used glues extensively; mosaic floors and tiled walls and baths are still intact after thousands of years. Around 1750, the first glue or adhesive patent was issued in Britain. The glue was made from fish. Patents were then rapidly issued for adhesives using natural rubber, animal bones, fish, starch, milk protein or casein. As we can see from ancient and medieval records, fish glue was both a common and important adhesive for many special applications; adapted by artists, it was used from the time of ancient Egypt to twentieth-century France, in painting media, coatings and grounds, in the gilding of illuminated manuscripts, and in pastel fixatives. 2)Theory of milk glue Casein is the principal protein found in cow milk (about 3%) from which it has been extracted commercially for most of the 20th century. It is responsible for the white, opaque appearance of milk in which it is combined with calcium and phosphorus as clusters of casein molecules, called micelles. The major uses of casein until the 1960s were in technical, non-food applications such as adhesives for wood, in paper coating, leather finishing and in synthetic fibers, as well as plastics for buttons, buckles etc. The first step in making acid-precipitated casein is acidification. Mineral acids or organic acids can add to the skim milk; or the milk is allowed to sour, in which case lactic acid, produced by fermentation of lactose, is the effective precipitant. The precipitated curd is then drained, washed, press to remove water, milled, drill, and ground. Solutions of casein in alkalis (with enough of the protein to give a suitable viscosity) can be used as glue. Such glue compares favorably in strength with animal glue on drying the protein forms numerous hydrogen bonds with the cellulose in paper and with itself, so it can stick woods together, but it is not water-resistant. Resistance to water can be imparted to casein glue, however, by modifying the simple formula of casein in alkali; these improved casein glues are widely useful in industry. Prepared casein glues are sold in the form of dry mixtures, which need only the addition of water before use. They are commonly composed of casein, lime, and a number of alkaline salts. Various chemicals have been used to improve the resistance to water, and many colloidal materials with adhesive properties can be mixed with casein to modify the properties of the resulting glues. It has thus been possible to adapt casein glues to a variety of specialized applications. These glues are used in the woodworking industry, in gluing paper, and in many other fields. Procedure à ¯Ã‚ ¿Ã‚ ½Preparation of milk glue 1) 250cm3 of milk and 50cm3 of vinegar (about 5% ethanoic acid) were added into a beaker. 2) The mixture was heated slowly and stirred continuously until curds were observed. (Caution: Heater is very hot, temperature of mixture should not be more than 60à ¯Ã‚ ¿Ã‚ ½C) 3) Curds were allowed to settle to the bottom for about 10 minutes. 4) The heated mixture was filtered. 5) The curds were squeezed to remove liquid. 6) The curds were washed with little volume of water and squeezed again. 7) The curds were stirred with solid sodium hydrogencarbonate (NaHCO3) gradually and monitored pH by pH paper until pH became slightly alkali. à ¯Ã‚ ¿Ã‚ ½Testing of adhere strength of glue 1) The glues were used to stick two plywood together, a the plywood at downside , with contact surface of 20 cm2 2) Two samples were made for each glue and one sample of starch glue was made as control. 3) The stack plywood was pressed by 0.8kg mass and allowed to dry at dry place for 2 days . 4) The plywood was set above the ground. 5) 0.5 kg Test mass was added gently each time until the masses drop. 6) The maximum mass of the glues that can afforded were recorded. 7) The plywood was cut into 4, which contact surface was decreased 4 times, and the above tests were repeated. Reference 1)http://classes.ansci.illinois.edu/ansc438/milkcompsynth/milkcomp_protein.html 2)http://science-in-farming.library4farming.org/Crops-Grains-Protein/PROTEINS-ARE-BASIC/Milk-Proteins.html

The Wrongful Conviction and Exoneration of David Milgaard :: Criminal Justice Essays

The Wrongful Conviction and Exoneration of David Milgaard Background At the time of the murder of which David Milgaard was accused of committing he was just 16 years old. He was a hippie, constantly in trouble. Even before he was a teenager he was getting into trouble. His parents and teachers considered him impulsive; he resisted authority (Regina Leader Post, 1992, as cited in Anderson & Anderson 1998). He was removed from kindergarten because he was considered to be a negative influence on the other children. When he was thirteen he spent time in a psychiatric centre (Anderson & Anderson, 1998) Elements of the Crime Gail Miller was a 22-year-old nursing assistant living in Saskatoon. She was found in an alley way between 6:45 and 7:30am on January 31st 1969. She had been raped, stabbed twelve times and left for dead. The rape was found to have occurred after she died. The police had little evidence; few clues had been left behind. There had been other attacks in the same area. Authorities tried to suppress the information that linked the Miller rape and murder to the two other assaults. Milgaard's Whereabouts David had decided to take a trip out to Alberta, along with two of his friends, Ron Wilson and Nichol John. Along the way there David wanted to stop through Saskatoon to pick up another friend, Shorty Cadrain. Once they got to Saskatoon they drove around not really knowing where they were going. They stopped to help someone out of a snow bank, and got stuck themselves; this is when Milgaard ripped his pants, something that came into evidence against him. When they finally arrived at Cadrain's home, David changed his pants, and they left to go to Alberta. Larry Fisher, who was later convicted of this crime (Miller's murder) was staying in the same home as Cadrain at the time. Some time after returning from their short trip to Alberta, Cadrain heard about the $2000 reward for information into Miller's death. Cadrain went to the police with a version of what happened that night, although he had been questioned earlier and had no information to give at that time. This is what made Milgaard a suspect in the case. From then on the police concentrated their efforts on finding evidence implicating Milgaard. Within the statement Cadrain gave to the police he said Milgaard had blood on his clothes, he threw away a woman's cosmetic case from the car on the way to Alberta and Cadrain also stated that he threatened to kill Wilson and John because they knew too much.

Biblical Archaeology: Dating Techniques

As our civilization grows, there is also a growing passion for knowledge of the past. Archaeology is fast becoming one of the popular sciences. Most archeological finds are composed of shreds of pottery or ceramics. As archeology and modern science progress many dating techniques are being developed to be used on these fragile artefacts in order to give us a brief glimpse of our past. Many techniques are now available which allow the detailed physical and chemical characterization of ceramic artefacts.Given a suitable archaeological sample it is now possible to determine many aspects of technology, provenance, and, in some cases, its use. Several dating techniques used in archeology are superposition, artifacts of known age, stratigraphy, dendrochronology and thermoluminescence. This paper shall focus on Ceramic Analysis using three of these techniques: Dendrochronology, Radiocarbon dating and Thermoluminescence. Carbon 14 Radiocarbon dating or Carbon-14 dating is the determination o f the approximate age of an ancient object, such as an archaeological specimen, by measuring the amount of carbon 14 it contains.Radiocarbon dating was discovered by a team of scientists led by Prof. Willard Libby of the University of Chicago after World War II. Since plants and animals take up carbon-14 during their lifetime. The amount of carbon-14 in them exists in equilibrium with the C14 concentration of the atmosphere and the ratio of C-14 to C-12 remains about the same as the ratio in the atmosphere. As soon as a plant or animal dies, they cease the metabolic function of carbon uptake; there is no replenishment of radioactive carbon, only decay.This rate of decay was calculated by Libby’s team to be 5568 years per half-life, this means that half the C14 in the original sample will have decayed after 5568 years and after another 5568 years, half of that remaining material will have decayed, and so on. This rate of decay is what is now know as Libby’s half-life wh ich he measured at 5568 ±30 years. The true advantage of the radiocarbon method is its capability to be uniformly applied throughout the world , this is probably the primary reason why it is one of the most widely used and best-known absolute dating methods.However, it is not without its flaws, radiocarbon dating has the following limitations: 1. that after 10 half-lives there is a very small amount of radioactive carbon present in a sample, at about 50,000-60,000 years radiocarbon dating becomes inaccurate. 2. the ratio of C-14 to C-12 in the atmosphere is not constant. This variation is due to changes in the intensity of the cosmic radiation bombardment of the Earth, the 1950’s nuclear testing and the depletion of the Ozone layer.3. in rare cases, a â€Å"reservoir effect† will give C-14 dates that are much older than the true age of the sample because â€Å"some plants and animals live in very unusual environments whose C-14 content is much lower than normal. â⠂¬  4. Contamination of the sample also limits the accuracy of Radiocarbon dating as when porous samples contain recently living material with a full â€Å"charge† of C-14. Finally, Radiocarbon dating can only be used on living organisms. DendrochronologyDendrochronology is the science that uses tree rings dated to their exact year of formation to analyze temporal and spatial patterns of processes in the physical and cultural sciences. Its main function in archaeology is to use tree rings to date when timber was felled, transported, processed, or used for construction or wooden artefacts such as a beam or pole of an ancient structure. The principle behind using tree-rings is that â€Å"tree-ring sequences from trees that grow in a seasonal climate, i. e., with one growth increment per year, with the size of that growth dependent upon some climatic stimulus such as cold in the Polar regions, drought in the Aegean, and various combinations of the two stimuli in regions in bet ween, can be compared so that these increments, more popularly known as â€Å"rings,† can be dated to the calendar year in which they were formed. † Aside from the principle of using tree-rings, the use of Dendrochronology is governed by several other principles, these set of scientific rules must be adhered to in order for the result to be flawless.There is the Uniformitarian Principle which states that â€Å"physical and biological processes that link current environmental processes with current patterns of tree growth must have been in operation in the past,† the Principle of Limiting Factors which states that â€Å"rates of plant processes are constrained by the primary environmental variable that is most limiting,† The Principle of Aggregate Tree Growth which states that â€Å"any individual tree-growth series can be â€Å"decomposed† into an aggregate of environmental factors, both human and natural, that affected the patterns of tree growth over time,† The Principle of Ecological Amplitude stating that a specie of trees â€Å"may grow and reproduce over a certain range of habitats, referred to as its ecological amplitude,† the Principle of Sites Selection which states that â€Å"hat sites useful to dendrochronology can be identified and selected based on criteria that will produce tree-ring series sensitive to the environmental variable being examined,† the Principle of Cross-dating which states that â€Å"matching patterns in ring widths or other ring characteristics (such as ring density patterns) among several tree-ring series allow the identification of the exact year in which each tree ring was formed. † and the Principle of Replication which states â€Å"that the environmental signal being investigated can be maximized, and the amount of â€Å"noise† minimized, by sampling more than one stem radius per tree, and more than one tree per site,† the application of which is not limited to Dendrochronology only.The methodology used in Dendrochronology is cross-dating, or matching patterns of ring-growth from one tree to another and assigning rings to specific years, however, this possible only among trees growing in the same general climatic region. The good thing about dendrochronology on the other hand, is that cross-dating can sometimes be achieved in spite of human interference to ring-growth such as thinning of stands, resin-gathering, fire damage, and other traumas such as severe weather effects, pollution or lightning damage, not to mention shaping of the wood at the time of construction and decay afterward. Furthermore, visual and statistical techniques are employed to guarantee the accuracy of the matches and in addition to simple ring-width analysis, X-ray densitometric methods are used to reconstruct past environmental conditions.It is also possible that wood or charcoal samples taken from standing buildings or excavated from archaeological site s be crossdated with each other and with wood from living trees to extend the tree-ring chronology beyond the date of the oldest ring of the oldest living tree in the region. Scientists believe that the best advantage of dendrochronology is that it is the only archaeometric technique where determination of absolute dates accurate to the year is either theoretically or practically possible, but, just like any other method of dating artefacts, it suffers from several limitations. The following limits the use or accuracy of Dendrochronology: 1. in some areas of the world, particularly in the tropics, the species available do not have sufficiently distinct seasonal patterns that can be used 2.in cases where the right species are available, the wood must be well enough preserved that the rings are readable to the point that there must be at least 30 intact rings on any one sample. 3. in order to produce an accurate result, it is necessary to have samples of timber that retained their bar k, so that it is clear which ring was the outermost when it was felled. 4. it can only be used if there is an existing master strip for that area and species; if the only master chronology available for the region is oak, cross-matching with timbers of these other species cannot be relied on. 5. use of this technique is also limited on how far back in the past things can be dated with tree rings although bristle cone pine trees can live to 9,000 years, this is a very rare phenomenon. 6.â€Å"sapwood is highly susceptible to decay particularly by beetle larvae and as a â€Å"result all sapwood may have been removed from the accessible surfaces of timbers during building repairs and conservation work, making it impossible to determine when the timber was felled. † 7. it may give inaccurate results on the actual date of the structure if it so happens that the date the timber was felled is not necessarily the date that the building was constructed or that the timber used was imp orted from another area. 8. in some places, prehistoric people may have built their structures using timber however, in most of the world that did not begin to happen until about 4,000 to 5,000 years ago.Thermoluminiscence Thermoluminescence dating is the determination by means of measuring the accumulated radiation dose of the time elapsed since the material containing crystalline minerals was either heated or exposed to sunlight. The application ranges from â€Å"Lower Paleolithic to Neolithic archaeological sites, with a major focus on the Middle Paleolithic, which is often beyond the range of the radiocarbon method. † The principle behind this unique dating technique is based on the â€Å"storage of information about the absorbed radiation in inorganic crystals. † It is based on structural damage and faults to the crystal lattice of minerals by ionizing radiation.The sources for this omnipresent radiation are radioactive nuclides from the surrounding sediment and f rom the sample itself, as well as secondary cosmic rays. Thus a radiation dose accumulates in the crystal in the form of electrons in excited states, of which some are metastable and thus resident over periods of time long enough to allow a dating application. During the first heating of the artefact, if the temperature is high enough (400 ° C), the drainage is sufficient to relax all electrons relevant to the luminescence method used; that is, the clock is set to zero and through the years it starts to accumulate natural radiation, upon its second heating it releases all this stored radiation in terms of thermoluminescent light.The released light is then correlated to the absorbed radiation which is then correlated to the archeological age. In practice, knowledge of the composition of the artefact is of importance since the nature or chemical composition of it, as explained above, determines the amount of natural radioisotopes present in it. These natural radioisotopes are respon sible for the greater part of the radiation that is absorbed. If the composition of the artefact is known, then the archeological age of the artefact can be computed by using the â€Å"total amount of absorbed radiation divided by the absorbed dose through internal and external radiation per year. †The greatest advantage of Thermoluminescence dating over other methods is not only the direct association of the event with past human activity on a linear time scale, but also its smaller vulnerability to unknown variation of certain parameters. However, Thermoluminescence dating is still prone to errors and inaccuracies. The evaluation and publishing of results must be done with due care and must meet with certain standards which includes the presentation of glow curves, heating- and DE-plateaus, growth curve(s) and the determination of the alpha sensitivity of each sample. Also, equal care has to be taken in the evaluation of parameters prone to variation with time. ConclusionEa ch of the three dating techniques discussed has its own peculiar way of finding the archaeological era that a sample was created or may have died: Radiocarbon depends on decay, Dendrochronology depends upon growth and thermoluminescence depends upon absorption. The three techniques however different they may be are still connected by a linear timeline, when the accuracy of one technique ends, another begins. Dendrochronology can only be used as long as there are master strips which may reach only up to a maximum of 9,000 years old, results obtained through Radiocarbon, on the other hand, can only be accurate up to 50,000-60,000 years old while Thermoluminescence dating ranges from 10,000 to 230,000 years old. The three dating techniques discussed have their own advantages and disadvantages.In the end, the scientist is the one who weighs all the pro’s and the con’s of any technique and he is given the discretion on what technique to use which he thinks is more appropria te and shall give out more accurate findings. BIBLIOGRAPHY Berger, Thomas. Thermoluminescence Dating: A Brief Overview. http://www. ati. ac. at/~vanaweb/papers/archview. pdf Fagan, Brian M. and George H. Michaels. Dating Techniques in Archaeology. http://www. mc. maricopa. edu/dept/d10/asb/anthro2003/archy/dating/datingtech. html#Dendrochronology Freestone, Ian. Ceramic Analysis. http://ads. ahds. ac. uk/catalogue/adsdata/cbaoccpap/pdf/117/11710001. pdf Grissino-Mayer, Henri D. Principles of Dendrochronology. http://web. utk. edu/~grissino/principles. htm Higham, Thomas. The Method. http://www. c14dating. com/int. html Hirst, Kris K.Radiocarbon Dating Method. http://archaeology. about. com/od/rterms/g/radiocarbon. htm Kuniholm, Peter Ian. Dendrochronology. http://www. arts. cornell. edu/dendro/ajatext. html Richter, Daniel. Advantages and Limitations of Thermoluminescence of Heated Flint from Paleolithic Sites. http://www. eva. mpg. de/evolution/staff/richter/pdf/07-RichterGeoarchae ology. pdf Robinson, B. A. How does Carbon-14 Dating (C-14) Work? Is it Accurate and Reliable?. http://www. religioustolerance. org/c14dats. htm Taylor, Jonathan. Dendrochronology in Dating Timber Framed Building and Structures. http://www. buildingconservation. com/articles/dendrochron/dendro. htm

Effects of British Colonial Rule in India Essay

The colonization of India and the immense transfer of wealth that moved from the latter to Britain were vital to the success of the British Empire. In fact, the Viceroy of British India in 1894 called India â€Å"the pivot of our Empire †¦Ã¢â‚¬  I examine the effects of the Industrial Revolution on the subcontinent. Besides highlighting the fact that without cheap labor and raw materials from India, the modernization of Britain during this era would have been highly unlikely, I will show how colonial policy led to the privation and death of millions of natives. I conclude that while India undoubtedly benefited from British colonial rule, the negatives for the subject population far outweighed the positives. . Colonialism, by definition, is exploitative and oppressive, with the rulers enriching themselves at the expense of those they rule. Generally speaking, colonizers dominate a territory’s resources, labor force, and markets; oftentimes, they impose structures — cultural, religious and/or linguistic — to maintain control over the indigenous population. The effects of the expansion of European empires, which began in the 15th century, on the colonized can still be felt today. Some historians, for example, argue that colonialism is one of the leading causes in income inequality among countries in present times. They cite patterns of European settlement as determinative forces in the type of institutions developed in colonized countries, considering them major factors in economic backwardness. Economist Luis Angeles has argued that the higher the percentage of Europeans settling in a colony at its peak, the greater the inequality in that country so long as the settlers remained a minority, suggesting that the colonizers drained those lands of essential resources while reaping most, if not all, of the profits. In terms of per capita GDP in 1995, the 20 poorest countries were all former colonies, which would seem to bolster Angeles’ contention. There are, however, competing views on how much underdevelopment in today’s poorest countries is a byproduct of colonial rule and how much of it is influenced by factors such as a country’s lack of natural resources or area characteristics. For poet, activist and politician Aime Cesaire, the verdict was in: Colonizers were â€Å"the decisive actors †¦ the adventurer and the pirate, the wholesale grocer and the ship owner, the gold digger and the merchant, appetite and force, and behind them, the baleful projected shadow of a form of civilization which, at a certain point in its history, finds itself obliged, for internal reasons, to extend to a world scale the competition of its antagonistic economies. This is not to suggest that Western European nations were the first and only countries to pursue imperialistic policies or that nothing good came out of colonial policies for the subject population. Dinesh D’Souza, while arguing that colonialism has left many positive as well as negative legacies, has stressed that there is nothing uniquely Western about colonialism, writing: â€Å"Those who identify colonialism and empire only with the West either have no sense of history or have forgotten about the Egyptian empire, the Persian empire, the Macedonian empire, the Islamic empire, the Mongol empire, the Chinese empire, and the Aztec and Inca empires in the Americas. † For this paper’s purposes, however, I will focus on the British Empire, its colonizing efforts in India (1757-1947), and the effects British policy had on that subject population. A couple of caveats before examining the British-Indian relationship: experiences differed from colony to colony during this period of European imperialism; India was unique in the colonial experience because of its size and history. It also should be noted that India was rather unique among colonized lands during this era for at least two reasons. First, South Asia was â€Å"already a major player in world commerce and possessed a well-developed trading and financial world† by the time Europeans arrived. Indigenous administrative structures already existed for taxation purposes, while commerce within the country and throughout the continent offered prospects of giant profits. Second, British India, which included today’s India, Pakistan and Bangladesh, was a region so large that there were areas in which Britain exercised direct control over the subject population and others where it exerted indirect control. It is exceedingly difficult, therefore, to extrapolate from one experience to another. Although it is impossible to determine how India would have developed had England never established a dominating presence there, I find the results of British colonialism to have been a mixed bag for India: the negatives, however, far outweighed the positives. Liberal and democratic aspects of British colonialism in India played a significant role in leading to a democratic South Asia following Indian independence in 1947. Yet, the British — first through the East India Company and then through direct government control — held almost all of the political and economic power in India during the Empire’s expansion and apogee, guaranteeing the Indian economy could not evolve and/or function independent of the ruling power’s control; ensuring raw materials extracted from Indian soil would go towards British manufacturing industries mostly without profiting the vast majority of Indians; and leading to lives of privation for millions of indigenous subjects. Although there have been arguments made that, in political and economic terms, south Asia was backwards until the arrival of Europeans, recent research has debunked that myth, showing the region to have possessed healthy trading and financial structures prior to the Europeans’ arrival. British Colonial Strategy in the Subcontinent Imperial powers followed two basic strategies when colonizing. They either allowed a large number of Europeans to settle overseas (known as Settler Colonies) or sent a much smaller number – usually less than 1 percent of the population — to serve as administrators and tax collectors (known as Peasant Colonies). Britain followed the latter strategy in regards to India. The percentage of English people in India in 1913, for example, was only 0. 1 percent of the country’s population; by comparison, they accounted for over one-fifth (21. 4 percent) of the population in South Africa and Losetho during the same period. As previously mentioned, Britain exerted both direct and indirect control over the Indian subcontinent. Areas of indirect control are called â€Å"native states. These were controlled by Indian rulers who wielded considerable power over the internal administration of the land, while the British exercised complete control over the area’s defense and foreign policies. When looking at this two-pronged approach Britain took in establishing an Indian colony, the economist Lakshmi Iyer has argued that there is a differential long-term effect on areas the Empire controlled directly compared to areas in which it basically outsourced control. Rather than expropriating Indian land, which was negligible, the English taxed Indian land, producing considerable revenues and inducing the indigenous population to shift from traditional to commercial products (e. g. tea). Areas that were directly under British control today have significantly lower levels of public goods relative to areas that were not under direct colonial rule. In 1961, for example, districts (administrative divisions below state level) that had been under direct control of the British Empire had lower levels of primary and middle schools, as well as medical dispensaries. Present-day differences between directly and indirectly controlled areas, Iyer argues, are most likely the result of differences in internal administration during the colonial period because once the British left in 1947, all the native states were integrated into independent India and have since been subject to a uniform administrative, legal and political structure. The Company and the Crown By the middle of the 18th century, there were five major European colonial powers — the Dutch Republic, France, Great Britain, Portugal, and Spain. From about 1850 on, however, Britain’s overseas empire would be unrivaled; by 1901, the empire would encompass 11. 2 million square miles and rule about 400 million people. For much of the 19th and 20th centuries, India was Britain’s largest and economically most important colony, an â€Å"empire within an empire. † It should be noted that although this period coincided with the birth of the Industrial Revolution historians and economists have cast doubt on whether industrialization was the sine qua non for British imperialism. They have noted that England’s first major advance into the Indian subcontinent began in Bengal in the middle of the 18th century, long before large-scale mechanization turned Britain into the â€Å"workshop of the world. † Historian P. J. Marshall, in studying early British imperialism, has written: â€Å"As a blanket term the Industrial Revolution explains relatively little about British expansion in general at the end of the eighteenth century. † While Marshall and others may be correct in asserting the British would have pursued empire even without the Industrial Revolution, its advent impacted colonial policy in that it required expanded markets and a steady supply of raw materials to feed the country’s manufacturing industries. Cotton, for example, was one of the driving forces behind the evolution of Britain’s modern economy. British traders purchased raw cotton fibers from plantations, processed it into cotton cloth in Lancashire mills, and then exported them to the colonial markets including India. Prior to the Industrial Revolution, India had been the world’s main producer of cotton textiles, with a substantial export trade. By the early nineteenth century, however, Britain had taken over dominating the world market for cotton textiles based on technology that lowered production costs . â€Å"This dramatic change in international competitive advantage during the Industrial Revolution was surely one of the key episodes in the Great Divergence of living standards between Europe and Asia. † Britain’s 200-year run ruling India began in the mid-17th century when the British East India Company set up trading posts in Bombay, Madras and Calcutta. In 1757, Robert Clive led Company-financed troops – led by British officers and staffed by native soldiers known as sepoys — in a victory over French-backed Indian forces. The victory at the Battle of Plassey made the East India Company the leading power in the country. It would dominate India for just over 100 years, the area it controlled growing over that time to encompass modern Bangladesh, a majority of southern India and most of the territory along the Ganges River in the north of the country. The East India Company’s control of Bengal alone yielded taxes of nearly  £3 million; by 1818, its territorial revenues in India stood at  £22 million, allowing it to finance one of the world’s largest standing armies. This established British rule well before the Industrial Revolution could have played any major role in Britain expanding its overseas empire, strengthening historians’ – Marshall, et al. – arguments regarding the significance, or lack thereof, of the role mechanization in England had in the country’s expansionist efforts. The fact remains, however, that Britain in the 19th century would become the world’s leading industrial power and India a major source of raw materials for its industry. What’s more, the subcontinent’s population of 300 million would constitute a huge source of revenue and a gigantic market for British-made goods. Although, the English expanded gradually in India during those first 100 years of colonization, once the British government gained control of the country’s administration following the Indian War of Independence in 1857, India was virtually incorporated into the British Empire and became its â€Å"crown jewel. † During the life of the Britain Empire, India was its most profitable colony. Examples of huge returns on British investments in India based on surviving business records are plentiful. To give two examples: Binny and Co. , which was founded in 1799 with 50,000 rupees in capital, returned profits of 140,000 rupees only 12 years later; and William Mackinnon’s Indian General Steam and Navigation Co. , which began trading in 1847 and whose assets five years later were valued at more than nine times the original capital of 72,000 rupees. The 1852 prospectus of the Chartered Bank of India, Australia, and China stated that â€Å"bearing in mind the very high rate of interest which prevails in the East and the very lucrative nature of the Exchange Business †¦ a very large Annual Dividend may be looked for with certainty. British investment in India increased enormously over the second half of the 19th and the beginning of the 20th centuries. According to economist James Foreman-Peck, by the end of 1911, 373 stock companies were estimated to be carrying on business exclusively or almost exclusively in India, yet were registered elsewhere, with the average size of those companies (railways accounted for nearly half of the capital, and tea plantations about one-fifth) dwarfing the far more numerous – 2,463 — Indian-registered companies. The discrepancies between the two are stark. The companies registered outside India had paid-up capital of â‚ ¤77.979 million and debentures of â‚ ¤45.353 million compared to â‚ ¤46.251 million and â‚ ¤6 million, respectively, for Indian-registered companies. According to Foreman-Peck, â€Å"The magnitude of foreign investment and the rate of return on it, broadly defined, have been seen as a means by which empire imposed burdens on colonies and boosted the imperial nation’s economy. † This was not an idea that could only be gleaned in hindsight. Writing at the end of the 19th century, historian Brooks Adams wrote the following: â€Å"Probably since the world began no investment has yielded the profit reaped from the Indian plunder. The amount of treasure wrung from the conquered people and transferred from India to English banks between Plassey and Waterloo (fifty-seven years) has been variously estimated at from $2,500,000,000 to $5,000,000,000. The methods of plunder and embezzlement by which every Briton in India enriched himself during the earlier history of the East India Company gradually passed away, but the drain did not pass away. The difference between the earlier day and the present is that India’s tribute to England is obtained by ‘indirect methods’ under forms of law. It was estimated by Mr.  Hyndman some years ago that at least $175,000,000 is drained away every year from India without a cent’s return. † Plunder and Famine At the time Britain established its colony on the subcontinent, the Indian economy was based predominantly on agriculture. Iyer has shown that since the Indian economy was so dependent on farming, British annexation policy focused on acquiring land with the most agricultural potential, guaranteeing that land taxation would be the East India Company’s/British government’s biggest source of income throughout the colonial period. In 1765-66, the East India Company had collected â€Å"the equivalent of  £1,470,000; and by 1790-1791, this figure had risen to  £2,680,000. † To ensure the land-revenue system, known as â€Å"tax farming,† would continue to supply money to the East India Company’s treasury, the Company introduced the Permanent Settlement of Bengal in 1793, an agreement between it and absentee landlords, known as zaminders. Through this policy, peasants who worked the land became the tenants of the zaminders, who, for themselves and the tax collectors, extracted as much as possible from those who cultivated the land. This settlement created a class of Indian landowners loyal to the English and a division in the rural society between the tenants and landlords, which last well into the 20th century. Indian climate is characterized by the monsoon, which generally includes nine months of dry weather followed by three months of rains known as the monsoon. At least once in a decade, the monsoon fails to arrive and a drought occurs. Indians for centuries had set aside a portion of crops to ensure there would be adequate food in times of drought. This practice was so successful that between the 11th and 18th centuries, India experienced only 14 major famines; yet, from 1765-1858, when it was under East India Company control, India suffered through 16 major famines, followed by an average of one famine every two years under British Colonial Office rule from 1859-1914. Under British rule during the 18th century, over 25 million Indians died of famine between: 1 million between 1800 and 1825, 4 million between 1825 and 1850, 5 million between 1850 and 1875, and 15 million between 1875 and 1900 ; more than 30 million deaths occurred from famine between 1870 and1910. Why did tens of millions die from starvation under the East India Company and the British Raj? Why, comparatively speaking, did so many famines occur under Britain’s watch? Historian Laxman D. Satya argues the famines were price-induced and that timely government intervention could have prevented millions of deaths from starvation. State intervention was minimal, however; Lord Curzon acknowledged once that a famine in Indian excited no more attention in Britain than a squall on the Serpentine. Like other European imperialists in the late 18th century, Britain – first through the East India Company – followed a laissez-faire doctrine whereby government interference in the economy was anathema; in addition, famine later was seen as a natural way to control overpopulation. According to Satya, â€Å"†¦ any act that would influence the prices of grains such as charity was to be either strictly monitored or discouraged. Even in the face of acute distress, relief had to be punitive and conditional. † The powers that be also began using famine labor to build an infrastructure – railways, roads – ensuring that revenues would continue to increase, expenditures would be kept low; worst of all, the new infrastructure allowed for the exportation of grain that could have fed the starving. Studies have shown that even in years of official famine – Britain only recognized three periods of famine — there was never a shortage of food grains. The problem was that with prices for grains so high and wages stagnant, most people could not afford to buy them. As an example, during the Indian Famine of 1887-88, nearly 44 percent of total exports from Berar, one of the hardest hit provinces, were food grains. Between 1874 and 1903 the province exported an average over 40 tons of grain, and Satya has shown that this could have amounted for nearly 30. pounds of food per person. Historian and social commentator Mike Davis has cited even evidence that grains were exported to Europe for speculative trading while millions were dying of starvation. Since the primary concern for the government was maximizing returns on investments, it didn’t prioritize famine relief, considering those expenditures wasteful; therefore, relief camps were â€Å"deliberately kept in remote locations and beyond the reach of the physically weakened population. What’s more, people seeking relief were required to work on colonial projects as a condition for receiving food – as little as 16-22 ounces of food for a minimum of nine-10 hours of often grueling labor Fearing that Indian nationalists would take to the newspapers – in general, the government had a comparatively lax policy toward the press — the Raj implemented tight press control through various laws including the Newspaper Act of 1908 and the Indian Press Act of 1910. It’s important to note that despite these and other attempts at press censorship, a large number of vernacular newspapers were published throughout the country and played an integral role in creating a nationalist/political consciousness in India.

Role of casein hydrolysate manufacturing conditions on hydrolysate technofunctional properties Essay Example

Role of casein hydrolysate manufacturing conditions on hydrolysate technofunctional properties Essay Example Role of casein hydrolysate manufacturing conditions on hydrolysate technofunctional properties Essay Role of casein hydrolysate manufacturing conditions on hydrolysate technofunctional properties Essay Introduction 1.1 Casein Milk contains about 30-35g protein /L. Approximately 80 % of which are present in casein micelles, these are big spherical composites incorporating 92 % protein and 8 % low molecular mass constituents, chiefly inorganic salts, chiefly calcium phosphate. Caseins represent four cistron merchandises IÂ ±s1-casein, IÂ ±s2-casein, I?-casein and I?-casein. Figure 1.1.1 shows the different protein types and their concentrations in milk. ( Advanced nutrient Chemistry A ) ( Advanced Food Chemistry A ) The precise construction of the casein is micelle has been subjected to legion scientific studied. Several theoretical accounts have been proposed over the last figure of old ages in order to depict casein micelle behavior. Caseins have distinguishable countries of positively and negatively charged groups in their primary constructions ensuing in amphiphilic belongingss. The caseins are known as rheomorphic proteins as they have highly flexible molecular construction. The caseins are thought to hold IÂ ±-helical or I?- sheet constructions, once more this is merely from theoretical surveies no such constructions have been found in the caseins because they have yet to be successfully crystallised. The secondary construction is loose and lacks order due to the high figure of proline residues which cause the protein concatenation to flex in a peculiar manner. Casein micelles are really stable against heat denaturation. As there is no third there is considerable exposure of hydrophobic residues, these consequence in strong association reactions and makes them comparatively indissoluble in H2O. All the caseins are conjugated proteins, most with one or more phosphate groups which are esterified to serine residues. These phosphate groups are of import to the construction of the casein micelle as Ca binding of the caseins is relative to the phosphate content. Table 1.1.2 shows some of the physicochemical features of the casein micelles. ( University G ) Physicochemical Features of Casein Micelle Diameter 50-300nm Surface Area 8 ten 10-10 cm2 Volume 2 ten 10-15 cm3 Density 1.063 g cm-3 Molecular weight ( hydrated ) 1.3 ten 109 Da Fullness 4.4cm3 per g protein Hydration 2g H2O per g protein Water Content ( hydrated ) 63 % ( Handbook ) ( Table 1.1.2 ) 1.2 Sodium Caseinate Manufacture ( Fig 1.2.1 ) ( Handbook ) Figure 1.2.1 gives a basic representation of the fabricating procedure of Na caseinate. The procedure involves foremost the separation of milk and pick, followed by pasteurisation. The caseins are so precipitated either by the add-on of a coagulator such as rennet or by a decrease in pH to 4.6 ( its isoelectric point ) . The curdled protein is heated to organize a curd. The curd is so separated from the whey by filtration or centrifugation in combination with counter-current rinsing with H2O. The curd is so reacted with an base, eg Na hydrated oxide and so dried to organize a caseinate. ( Handbook ) 1.3 Bioactive Peptides The primary construction of proteins consists of certain aminic acid sequences that have the ability to exercise physiological benefits in human existences. The amino acid sequences remain inactive when they are present as portion of the uninterrupted primary construction of the parent protein. However, when the parent protein is acted upon by an appropriate enzyme, the peptide is released ( Dr Rotimi Aluko ) . Enzymatic hydrolysis of milk proteins has been shown to cut down antigenicity, and increase biological activity for illustration by the release of immunomodulating, opioid and antihypertensive peptides. To this terminal, turning involvement has been focused on physiologically active peptides derived from milk proteins. In add-on, the little peptides present in protein hydrolysates are absorbed more quickly from the bowel than free amino acids or integral proteins. ( Spellman et Al ) . A sum-up of bioactive peptides which are derived from milk proteins and their maps are displa yed in figure 1.3.1 ( Hannu K ) ( Fig 1.3.1 ) 1.4 Enzyme readying The enzyme readying used for casein hydrolysis in this survey was Prolyve 1000a„? a commercially available protease readying which is of bacterial beginning. This readying contains the enzyme Subtilisin Carlsberg which is from a household of serine endopeptidases isolated from Bacillus licheniformis. Endopeptidases are enzymes which cleave within the protein or polypeptide concatenation. Subtilisin Carlsberg has a wide specificity for the hydrolysis of peptide bonds, with a penchant for a big uncharged residue. It is an aromatic enzyme with a penchant for carboxyl side cleavage. Hydrolyzes peptide amides incorporating leucine and tyrosine residues. This enzyme readying has been used in the hydrolysis of whey proteins ( Spellman et Al, 200 ) nevertheless to day of the month at that place does non look to be any publications utilizing this enzyme activity in the hydrolysis of casein substrates. 1.5 Resentment The chief disadvantage of protein hydrolysis is bitterness. Integral nutrient proteins due to their molecular size are improbable to interact with the taste-bud receptors and as such would non lend significantly to season ( Enzymology ) . As hydrolysis interruptions down these proteins into much smaller peptides interaction with taste-bud receptors can happen. Besides in Intact casein micelles the most hydrophobic amino acids are oriented towards the inside of the molecule, during hydrolysis peptides incorporating hydrophobic amino acids are released. As hydrolysis continues, more hydrophobic amino acid residues become exposed for this ground hydrolysate resentment by and large increases with increasing hydrolysis ( Spellman et Al ) . The Q-rule devised by ( Ney, 1971 ) established a quantitative relationship between the amino acerb composing of a peptide and its resentment. Using the values calculated by ( Tanford,1962 ) , the Q-rule stated that peptides with an mean hydrophobicity ( Q ) value greater than 1400calmola?’1 and with molecular multitudes below 6000Da elicit a acrimonious taste. ( Lemieux,1992 ) . Figure 1.5.1 shows several protein types and their Q values, it besides shows the different casein categorizations and their single Q values. I?-casein has a Q value of greater than 1400 kcal mol-1 this may slightly predispose to bitterness if during the hydrolysis the proteins are broken down to peptides weighing less than 6000Da ( Figure 1.5.1 ) Bitter peptides typically contain 3-15 amino acids and are characterised by the presence of hydrophobic aminic acids such as leucine, isoleucine, proline, valine, phenylalanine, tyrosine and tryptophan. ( Enzymology ) . The distinguishable acrimonious spirit of protein hydrolysates has been a major restriction in their usage in nutrient and wellness merchandises, they would necessitate to be incorporated into nutrients at really low concentrations to forestall its presence bring forthing an unacceptable spirit. 1.6 Enzymatic Hydrolysis of protein and the factors which affect it Enzymatic hydrolysis of protein is the procedure by which proteins are broken down by peptidases. Several factors affect the rate hydrolysis these include, enzyme specificity, extent of protein denaturation, enzyme: substrate ratio, entire solids concentrations, viscousness, pH, ionic strength, temperature and absence or presence of repressive substances. The specificity of an enzyme is a cardinal factor, act uponing both the figure and location of the peptide linkages that are hydrolyzed. Endopeptidases cleave the peptide linkage between two next amino acid residues in the primary sequence of a protein, giving two peptides. Proteolysis can continue either consecutive, by let go ofing one peptide at a clip, or through the formation of intermediates that are farther hydrolyzed to smaller peptides. ( Panyam et al, 1996 ) . There is really small information available about the consequence of entire solids on the rate of hydrolysis or the resulting belongingss of the hydrolysate samples. Spellman et Al, 2004 carried out a survey on whey protein hydrolysates and how entire solids affected the rate of the hydrolysis and the physiochemical belongingss of the end point hydrolysates. They concluded that the resentment of the hydrolysate samples decreased with increasing entire solids concentrations. Viscosity is the step of opposition to flux. Solvent viscousness can act upon rates of enzyme catalyzed reactions by two principle mechanisms: ( 1 ) Since molecular diffusion coefficients vary reciprocally with the viscousness of the medium, an addition in solvent viscousness will take to a lessening in the association rate of an enzyme and substrate. This will attest itself in a viscosity-dependent lessening in kc/Km for reactions in which the procedure that is governed by kc/Km is diffusion-controlled. ( 2 ) Since dissolver viscousness dampens structural fluctuations of proteins through frictional effects, additions in solvent viscousness will take to lessenings in reaction rates for catalytic procedures that are dependent on enzyme structural fluctuations Dang, 1998 this is supported by surveies from Gavish, 1979 and Ng, 1991 The consequence of temperature on the rate of enzymatic hydrolysis relates to the enzyme and the optimal conditions to ease its reaction but besides to the substrate where cautiousness must be taken in order to avoid /facilitate heat denaturation. Whichever of the conditions is preferred should be controlled. pH besides affects the rate of enzyme action and may present a menace of denaturation the enzyme. pH besides has an consequence on protein solubility. The iso-electric point is the pH at which the protein has no net charge, at this pH proteins would lose solubility and precipitate out of solution. Enzymes work better on proteins in solution. The ionic strength of the may impact the rate of hydrolysis. A solution of low ionic strength ( 0.5M 1.0M ions of impersonal salts ) may increase the solubility of the protein by salting in . Whereas concentrations above 1.0M may cut down the solubility of the protein as it causes increased competition for H2O molecules, favoring protein- protein interactions and thereby salting out . 1.7 TNBS Assay The method used to quantify the grade of hydrolysis ( DH ) of the Na caseinate hydrolysates was the trinitrobenzenesulfonic acid ( TNBS ) assay that was described by Adler-Nissen,1979 ) . Degree of hydrolysis ( DH ) is defined as the per centum of the entire figure of peptide bonds in the protein which have been cleaved by hydrolysis. The TNBS Assay was used in this undertaking as it has been proven to be a extremely accurate method for quantifying the DH of hydrolysate samples ( Spellman, 2003 ) . The one drawback of this method is that is requires long incubation and chilling stairss. This method is a spectrophotometric check of the chromophore formed by the reaction of the TNBS with primary Zanzibar copals. Figure 1.8.1 is a representation of the basic mechanism of how the TNBS check plants. Simply put the TNBS reagent binds to the NH2 group of the peptides in the sample and in making so creates a xanthous coloring material. The more hydrolyzed the sample is the more peptides there are and hence the more NH2 groups to adhere to and the more xanthous the solution will go. These solutions are so read spectrophotometrically . This check consists of several stairss foremost the protein hydrolsate is dispersed in hot 1 % Na dodecyl sulphate ( SDS ) , this serves to guarantee an accurate consequence as it prevents clip-clop of the protein and enables the TNBS reagent to split all of the NH2 groups. This reaction favours somewhat alkalic conditions ( pH 8.2 ) which is facilitated by the add-on of Na phosphate buffer. TNBS reagent reacts easy with hydroxyl ions and could do the space to give a false somewhat increased reading. This addition is stimulated by visible radiation and for this ground many of the stairss are carried out in the dark, such as the incubation for 1hour at 50Â °C and the subsequent expiration of the reaction which is achieved by take downing the pH. Termination is accomplished by the add-on of HCL, cautiousness must be taken to guarantee that the pH does non drop at a lower place 3.5 as this would causes turbidness. The samples are allowed to chill at room temperature for 30mins, chillin g below room temperature may besides do turbidness. After standing for 30mins the samples and their optical density readings are stable and more accurate. 1.8 Reverse Phase -High Performance Liquid Chromatography ( RP-HPLC ) Reverse-phase ( RP ) HPLC is an indispensable tool in the separation of proteins and peptides. RP-HPLC is widely used in protein surveies because of its versatility, sensitive sensing ( can divide proteins of about indistinguishable construction ) and its ability to work together with techniques such as mass spectroscopy. High public presentation liquid chromatography severs to heighten detectability of the analyte and can be applied to the analysis of any compound with solubility in a liquid that can be used as the nomadic stage ( Rounds, 1988 ) . The major constituents of a high public presentation liquid chromatography system include a pump, injector, column, sensor and information system. Change by reversal stage -HPLC is where the mutual oppositions of the stationary stage and the nomadic stage are reversed in comparing to the normal stage of soaking up chromatography ( Macrae, 1988 ) . The stationary stage is a solid support that is non-polar. Reversed stage media are composed of a base matrix to which organic ligands for surfacing silicon oxide such as octyl ( C8 ) or octadecyl groups ( C18 ) are attached ( Sofer, 1997 ) . The nomadic stage is a polar liquid that flows over the stationary stage. The sample is dissolved in the initial nomadic stage ( eg. Trifluoroacetic acid ) prior to being filtered and applied by injection to the column. Polar nomadic stages are normally H2O assorted with methyl alcohol, acetronitrile or trifluoroacetic acid ( Rounds, 1988 ) . The interaction of the constituents bring separated and the stationary stage rely on hydrophobic interactions and this determines the grade of migration in the column and separation of the constituents in the sample. Polar compounds are the first to be eluted as they are hydrophilic and have hebdomad interactions with the stationary stage. The pumps map is to present the nomadic stage through the system at a controlled flow rate of 1ml/min. Gradient elution system is used which involves two independent programmable pumps that are mixed at high force per unit areas ( Rounds, 1988 ) . This allows different compounds to be eluted by increasing the strength of the organic dissolver in a additive manner. The usage of a valve injector places the sample for dividing into the following nomadic stage and it is carried in this liquid for debut into the column. The sample injection is normally automated. The HPLC column is normally constructed of chromium steel steel tubing with eradicators that allow it to be connected between the injector and sensor of the system ( Rounds, 1988 ) . The packing stuff for this column is in the signifier of a chromatographic bed and acts as both a stationary stage and a support. The sensor for the HPLC is the constituent that emits a response due to the eluting sample compound/ concentration alterations in the column eluent and later signals a extremum on the chromatogram. Peptides do non absorb light above 220nm and soaking up of 214nm may be used to follow the concentration of peptides in the column wastewater ( Sofer, 1997 ) . Optical density of peptide at this wavelength is performed as peptides do non hold a 3-dimensional construction and all the amino acids are exposed and easy interact with the chromatographic media. Proteins don non hold the same interaction with the media as merely a little measure of its molecules tend to interact. Proteins usually show an optical density at 280nm due to the content of aromatic amino acid replacements ( Sofer, 1997 ) . Ultra violet sensing allows following of protein concentration of the wastewater and a chromatogram visually shows the extremums of the peptides and aromatic amino acids nowadays in sample. Column and nomadic stage temperature and pH can impact the separation of proteins and peptides by RP-HPLC. Increasing the temperature reduces the keeping of peptides. The temperature affects the comparative keeping of selectivity, which affects declaration ( David Carr ) . 1.9 Centripetal Evaluation Centripetal rating involves both rules of experimental design and statistical analysis. Centripetal rating of nutrient can utilize the human senses of gustatory sensation, touch, sight and odor to measure different facets of nutrient such as spirit, texture, visual aspect and olfactory property. For this undertaking the accent is on spirit and gustatory sensation will be the sense of pick. Flavour can be referred to as the esthesis perceived from nutrient or liquid taken in the oral cavity ( Fisher et al, 1997 ) . The four basic gustatory sensations are sweet, rancid, salty and bitter. For this undertaking a centripetal panel was selected and trained. Campaigners for the panel were selected foremost on the footing of being able to separate between Sweet, rancid, salty and bitter. Successful campaigners were so trained to observe and quantify resentment utilizing caffeine criterions. Statistical analysis of the resentment tonss was carried out utilizing the statistical programme R ver sion 2.10.1AÂ © , One-way analysis of discrepancy ( ANOVA ) and independent-samples t-tests were performed on centripetal informations. A important consequence was defined as P lt ; 0.05, a extremely important consequence was defined as P lt ; 0.001. Centripetal rating is a important facet in every undertaking whose ultimate end is to market a food/ functional nutrient merchandise. Bitterness is a major restricting factor in the production and incorporation protein hydrolysate into nutrients and centripetal rating is the most effectual method of finding the resentment degree. 1.10 Aims of this survey The aims of this survey are: O To bring forth Na caseinate hydrolysates at different protein/ entire solids concentrations. O Physicochemical word picture of the hydrolysate samples. O Selection and preparation of a centripetal panel to find the degree of resentment of the hydrolysates. O To find if the resentment of Na caseinate hydrolysates is related to protein/ entire solids concentration at which the hydrolysates were generated. Chapter 2 Materials and Methods 2 Materials and Methods 2.1 Materials Sodium caseinate was obtained from Kerry Group, Ireland and its protein content was determined to be 88.01 % utilizing Kjeldhal analysis. Prolyve 1000 was obtained from Lyven Enzymes Industrielles, Caen, France Trifluoroacetic acid ( TFA ) , HPLC class acetonitrile, L-leucine, HPLC grade H2O, Citric acid, Na chloride, sucrose and caffeine were obtained from Sigma Chemical Co. ( Poole, Dorset, UK ) . PuradiscTM 25 AS disposable syringe filters ( 0.2 AÂ µm ) , Supor_ hydrophilic membrane filters ( 47 millimeter, 0.2 lumen ) and 2N NaOH ( Titripur, SWR, 1.09136,1000 ) were obtained from VWR chemicals, Ireland. 2.2 Protein finding utilizing the Kjeldahl process The Kjeldhal method for finding protein concentration is an accretion of several reactions, the first of which is digestion, where protein N is liberated to organize ammonium ions. Sulphuric acid oxides organic affair and it combines with the ammonium formed. The 2nd reaction consists of the sulfuric acid in the sample being neutralised with NaOH organizing ammonium hydroxide which is so distilled into a 4 % boracic acerb solution for the 3rd reaction. For the forth reaction a titration takes topographic point between the borate ions formed and the standardized 0.1M HCL until a pH of 4.6 is reached. Oz Moles of HCL= Moles of NH3 = Moles of N in sample. % Nitrogen x transition factor ( 6.38 ) = % protein Approximately 0.2g of Na caseinate pulverization was accurately weighed out and transferred into a Kjeldhal flask. Sucrose was used as a space. Into each flask 20mL of concentrated H2SO4 ( Low in N ) and two kjeldhal tablets. The samples were so placed in the Kjeldhal digestion unit ( Buchi, Labortechnik AO, Postfrach, Switzerland ) at 120Â °C for half an hr and temp increased to 420Â °C for 2 hours. During this clip digestion of organic affair in the sample occurs. After digestion the tubings are so cooled before being transferred to the Kjeldhal Buchi B323 Distillation unit for distillment and subsequent finding of protein content. This process was performed in triplicate. 2.3 Enzymatic hydrolysis of Na caseinate For the balance of this thesis the hydrolysate samples will be referred to in respects to protein concentration. Table 2.3.1 shows protein concentration in proportion to entire solids concentration for the Na caseinate hydrolysates generated at different protein/ entire solids concentrations. Table 2.3.1 Protein Concentration Entire Solids Concentration 5 % ( w/v ) 56.8g/L 10 % ( w/v ) 113.6g/L 15 % ( w/v ) 170.4g/L 20 % ( w/v ) 227.2g/L The enzymatic hydrolysis experiments were carried out in a 2L certain reaction vas ( ___ ) . The aqueous solutions of Na caseinate were allowed to hydrate for ~ 2hours at 50Â °C with the assistance of an overhead scaremonger. The solutions were so stored in a electric refrigerator at 4Â °C nightlong. On the twenty-four hours of the hydrolysis the solutions were equilibrated to 50Â °C and the pH was so adjusted to 7 by the add-on of 2N NaOH before the add-on of the enzyme. Prolyve 1000 was added at an enzyme: substrate ( Tocopherol: S ) ratio of 25ml enzyme readying / kilogram protein. The Tocopherol: S ratio was estimated on the footing of what has antecedently worked for whey, the standard add-on was 0.25mls of enzyme solution per 100ml to 10 % solution. The solution was assorted with an over caput scaremonger ( Heidolph Instruments, Schwabach, Germany ) and the pH was kept changeless throughout the hydrolysis utilizing a pH stat ( 718 Stat Titrino, Metrohm, Herisau, Switzerland ) . Hydrolysate samples were taken at assorted clip intervals, rapidly brought to 80Â °C utilizing a microwave and so maintained at 80Â °C for 20mins in a H2O bath to demobilize the enzyme. The samples were so stored at -20Â °C until required for analysis. 2.4 Quantification of Degree of Hydrolysis ( DH ) The TNBS reagent was made up of 0.1 % ( w/v ) TNBS in H2O. 1 % ( w/v ) SDS was used as the space and l-Leucine was used as the criterion. Samples and standard solutions were prepared in 1 % ( w/v ) SDS. The hydrolysate samples were diluted ( 1 in 51, 1 in 76, 1 in 101, 1 in 126, 1 in 151 A ; 1 in 201 in conformity with their protein concentrations ) . All samples were done in triplicate. 0.25 milliliter of the trial, integral Na caseinate ( control ) and l-Leucine criterion solutions was added to prove tubings incorporating 2.0mL of Na phosphate buffer ( 0.2125 m, pH 8.2 ) . The undermentioned stairss are light sensitive and took topographic point in the dark: 2 milliliter of TNBS reagent was so added to each tubing, followed by vortexing and incubation at 50AÂ °C for 60 min in a covered H2O bath. After incubation, the reaction was stopped by the add-on of 0.1N HCl ( 4.0 milliliter ) to each tubing. The samples were so allowed to chill in the dark at room temperature in order to st abilise the optical density readings. The optical density values were so read at 340nm utilizing a ( Carey 100 dual beam spectrophotometer ) The DH was calculated as follows: Where the N content of peptide bonds = 112.1 milligram of Nitrogen /g of protein for casein substrates. The protein concentration became marginally more dilute as NaOH was added throughout the hydrolysis reaction ; the protein concentration was calculated consequently. 2.5 RP-H.P.L.C. Reversed-phase HPLC was carried out on the Na caseinate hydrolysate samples utilizing a Waters HPLC system, consisting a Model 1525 binary pump, a Model 717 Plus autosampler and a Model 2487 double IÂ » optical density sensor interfaced with a BreezeTM data-handling bundle ( Waters, Milford, MA, USA ) . The column used was a Phenomenex Jupiter ( C18, 250r4.6 millimeter ID, 5 millimeter atom size, 300AAÂ ° pore size ) dividing column ( Phenomenex, Cheshire, UK ) with a Security GuardTM system incorporating a C18 ( ODS ) broad pore cartridge ( 4r3 millimeter ID, Phenomenex, Cheshire, UK ) . The column was equilibrated with dissolver A ( 0.1 % TFA ) at a flow rate of 1.0 ml min-1 and peptides were eluted with an increasing gradient of dissolver B ( 0. 1 % TFA, 80 % acetonitrile ) . TFA is used in HPLC as it improves symmetricalness of signals. Detector response was monitored at 214 nanometer A ; 280nm. The Na caseinate hydrolysate samples were diluted to 0.8 % ( w/v ) in 0.1 % TFA, filtered through 0.2 AÂ µm syringe filters and 20 AÂ µl was applied to the column. The undermentioned tabular arraies show the 3 different gradient profiles used in analyzing the samples. Basic contrary stage high public presentation liquid chromatography gradient profile Time Flow % A % B Curve 1 0.01 1.00 100.0 0.0 6 2 4.00 1.00 100.0 0.0 6 3 54.00 1.00 40.0 60.0 6 4 55.00 1.00 0.0 100.0 6 5 65.00 1.00 0.0 100.0 6 6 70.00 1.00 100.0 0.0 6 7 85.00 1.00 100.0 0.0 6 ( Table 2.5.1 ) Modified Profile 1 Time Flow % A % B Curve 1 0.01 1.00 100.0 0.0 6 2 4.00 1.00 100.0 0.0 6 3 84.00 1.00 40.0 60.0 6 4 85.00 1.00 0.0 100.0 6 5 95.00 1.00 0.0 100.0 6 6 100.00 1.00 100.0 0.0 6 7 115.00 1.00 100.0 0.0 6 ( Table 2.5.2 ) Modified Profile 2 Time Flow % A % B Curve 1 0.01 1.00 100.0 0.0 6 2 4.00 1.00 100.0 0.0 6 3 114.00 1.00 40.0 60.0 6 4 115.00 1.00 0.0 100.0 6 5 125.00 1.00 0.0 100.0 6 6 130.00 1.00 100.0 0.0 6 7 145.00 1.00 100.0 0.0 6 ( Table 2.5.3 ) The ultimate profile used on the samples with similar DH values but of different protein concentrations was the modified profile 2. 2.6 Centripetal In choosing campaigners for the gustatory sensation panel a acknowledgment trial for the four gustatory sensations was foremost performed. This involved doing up solutions of citric acid monohydrate ( rancid ) , sucrose ( sweet ) , caffeine ( acrimonious ) and NaCl ( salt ) all of which were made up in Ballygowan still H2O. At least two concentrations of each sample stand foring a gustatory sensation were included in the centripetal rating see table 2.6.1 Recognition Test Layout Sample Letter Concentration Chemical ( Taste ) A 0.02 % ( w/v ) Citric acid monohydrate ( Sour ) Bacillus 0.40 % ( w/v ) Sucrose ( Sweet ) C 0.03 % ( w/v ) Citric acid monohydrate ( Sour ) Calciferol 0.02 % ( w/v ) Caffeine ( Bitter ) Tocopherol 0.08 % ( w/v ) NaCl ( Salty ) F 0.60 % ( w/v ) Sucrose ( Sweet ) Gram 0.03 % ( w/v ) Caffeine ( Bitter ) Hydrogen Ballygowan still H2O Joule 0.15 % ( w/v ) NaCl ( Salty ) K 0.04 % ( w/v ) Citric acid monohydrate ( Sour ) ( Table 2.6.1 ) The campaigners were told that the samples contained natural Sweet, salty, rancid and acrimonious compounds. They were asked to savor each sample separately and bespeak whether the sample was sweet, salty, rancid or bitter in the provided infinites. If the sample tasted like H2O they were asked to tag with a nothing ( 0 ) , if they were unsure of the gustatory sensation they were asked to tag with a inquiry grade ( ? ) . When savoring the sample the campaigners were asked to twirl the solution around your oral cavity guaranting it contacts all parts of the lingua. Between samples, campaigners were asked to eat a piece of un-salted cracker and rinse their oral cavities exhaustively with still mineral H2O. A cut off point was set at 6 right replies, 1 of which must be acrimonious or 5 right replies, 2 of which must be acrimonious. Out of the 17 campaigners screened, 7 were selected for bitterness preparation. For the resentment preparation campaigners were asked to delegate bitterness tonss to unknown solutions based on a 0-100 % graduated table, where a 100 % acrimonious solution was taken to hold a resentment value equivalent to 1 g caffeine/L. Still mineral H2O was used as the 0 % bitterness criterion. All 7 displayed a strong ability to observe differen